ITGB4 Antibody / CD104 Polyclonal Antibody (R31440)

ITGB4 Antibody in Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue with ITGB4 Antibody at 1 ug/ml. HIER was performed by boiling tissue sections in pH 6 10mM citrate buffer for 10-20 min followed by cooling before testing. Strong brown staining is concentrated along the epithelial glandular structures, with particularly prominent membranous and basal-associated signal surrounding the glandular spaces. The image demonstrates the epithelial localization expected for integrin beta 4 and tissue detection using the CD104 Polyclonal Antibody.

ITGB4 Antibody in Human Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue with ITGB4 Antibody at 1 ug/ml. HIER was performed by boiling tissue sections in pH 6 10mM citrate buffer for 10-20 min followed by cooling before testing. Distinct brown staining outlines portions of the chorionic villi, with signal concentrated predominantly along the peripheral trophoblastic cell layers rather than throughout the villous cores. This localized pattern demonstrates detection of integrin beta 4 with the CD104 Polyclonal Antibody.

ITGB4 Antibody Western Blot of Recombinant Human ITGB4. Western blot testing of recombinant human ITGB4 protein (0.5 ng) with ITGB4 Antibody. The blot shows a single strong band migrating at approximately 40 kDa. This is a recombinant ITGB4 fragment rather than full-length approximately 200 kDa integrin beta 4, so the lower molecular weight is expected for the recombinant protein preparation. The clean single-band result demonstrates recognition of the target protein by the CD104 Polyclonal Antibody.

ITGB4 Antibody Western Blot of A549, PC-3, T-47D, HeLa and Caco-2 Cells. Western blot testing of human A549 (lane 1), PC-3 (lane 2), T-47D (lane 3), HeLa (lane 4), and Caco-2 (lane 5) cell lysates with ITGB4 Antibody at 0.5 ug/ml. The predicted molecular weight is approximately 200 kDa. A prominent band near 180-200 kDa is detected in lanes 1, 2, 3, and 5, with much weaker signal in lane 4. Additional lower-molecular-weight bands are visible, particularly in lane 1. The major high-MW signal is consistent with full-length integrin beta 4 detection by the CD104 Polyclonal Antibody.

ITGB4 Antibody in Human Placenta IF. Immunofluorescence staining of FFPE human placenta tissue with ITGB4 Antibody at 1 ug/ml. HIER was performed by boiling tissue sections in pH 6 10mM citrate buffer for 10-20 min followed by cooling before testing. Green ITGB4 signal prominently outlines the peripheral cell layers of multiple chorionic villi, while nuclei are counterstained blue. The sharply localized peripheral staining corresponds well with the distribution seen independently by IHC and demonstrates detection using the CD104 Polyclonal Antibody.

ITGB4 Antibody in Human A431 Epidermoid Carcinoma Cells FACS. Flow cytometry testing of human A431 epidermoid carcinoma cells with ITGB4 Antibody at 1 ug/10^6 cells following blocking with goat sera. Cells alone are shown in red, isotype control in green, and ITGB4 antibody staining in blue. The blue antibody-treated population shows a substantial rightward fluorescence shift relative to the green isotype control, demonstrating strong detection of cell-associated integrin beta 4 by the CD104 Polyclonal Antibody.