ITGB2 Antibody 1A3/2A10 / CD18 Monoclonal Antibody [clone 1A3/2A10] (RQ4511)

ITGB2 Antibody 1A3/2A10 in Human Tonsil IHC. Immunohistochemistry testing of FFPE human tonsil tissue with ITGB2 antibody clone 1A3/2A10 at 2 ug/ml. HIER was performed by boiling tissue sections in pH 6 10mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. The image shows widespread heterogeneous brown staining among cells throughout the lymphoid tissue, with numerous strongly positive cells against more weakly stained areas. This pattern demonstrates tissue detection of integrin beta 2 using the CD18 Monoclonal Antibody.

ITGB2 Antibody 1A3/2A10 in Human Rectal Cancer IHC. Immunohistochemistry testing of FFPE human rectal cancer tissue with ITGB2 antibody clone 1A3/2A10 at 2 ug/ml. HIER was performed by boiling tissue sections in pH 6 10mM citrate buffer for 10-20 min followed by cooling at RT for 20 min. Brown staining is concentrated in numerous cells within the stromal regions surrounding the glandular tumor structures, while the epithelial structures show comparatively little staining. The distribution is consistent with CD18-positive infiltrating immune cells and demonstrates detection with the CD18 Monoclonal Antibody.

ITGB2 Antibody 1A3/2A10 Western Blot of Human Placenta. Western blot testing of human placental lysate with ITGB2 antibody clone 1A3/2A10 at 1 ug/ml. A discrete band is visible at approximately 85-90 kDa, within the expected 85-95 kDa range for glycosylated CD18. The blot is relatively clean, with the target-sized band clearly distinguishable from background signal. This result demonstrates detection of ITGB2 in human placenta by the CD18 Monoclonal Antibody.