ITGA3 Antibody / Integrin Alpha 3 Polyclonal Antibody (RQ4580)

ITGA3 Antibody by IHC in human esophageal squamous carcinoma. Immunohistochemistry of FFPE human esophageal squamous carcinoma using ITGA3 Antibody at 2 ug/ml. Strong brown immunoreactivity is visible throughout malignant squamous epithelial cells, with prominent membranous staining and additional cytoplasmic labeling. Adjacent connective tissue exhibits comparatively weaker staining. Hematoxylin counterstaining identifies blue nuclei. Antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight incubation at 4oC. This Integrin Alpha 3 Polyclonal Antibody demonstrates CD49c immunoreactivity in esophageal carcinoma tissue.

ITGA3 Antibody by IHC in human colon cancer. Immunohistochemistry of FFPE human colon cancer tissue using ITGA3 Antibody at 2 ug/ml. Brown DAB staining is prominent in malignant epithelial cells, with membranous and cytoplasmic immunoreactivity outlining glandular structures. Surrounding stromal regions show comparatively limited staining, while hematoxylin highlights blue nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Integrin Alpha 3 Polyclonal Antibody detects CD49c in human colorectal tumor tissue.

ITGA3 Antibody by IHC in human placenta. Immunohistochemistry of FFPE human placenta tissue using ITGA3 Antibody at 2 ug/ml. Brown DAB immunoreactivity is visible along the outer surfaces of chorionic villi, with prominent staining in trophoblast-associated regions. Hematoxylin counterstaining highlights blue nuclei within the villous structures. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Integrin Alpha 3 Polyclonal Antibody detects CD49c, a receptor involved in cell adhesion and extracellular matrix interactions.

ITGA3 Antibody by IHC in human testicular cancer. Immunohistochemistry of FFPE human testicular cancer tissue using ITGA3 Antibody at 2 ug/ml. Brown immunoreactivity is distributed throughout tumor-associated cells, with variable membranous and cytoplasmic staining. Hematoxylin counterstaining highlights blue nuclei and surrounding tissue architecture. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Integrin Alpha 3 Polyclonal Antibody demonstrates CD49c immunoreactivity in human testicular tumor tissue.

ITGA3 Antibody by IF in A431 cells. Immunofluorescence staining of human A431 cells using ITGA3 Antibody at 5 ug/ml. Green Fluoro488 fluorescence is visible along cell boundaries and throughout portions of the cytoplasm, consistent with the distribution of integrin alpha 3. Nuclei are counterstained blue with DAPI. Enzymatic antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used at 1:500. This Integrin Alpha 3 Polyclonal Antibody demonstrates cellular detection of CD49c.

ITGA3 Antibody by IF in human A431 tumor cell line. Immunofluorescence staining of FFPE human A431 cells using ITGA3 Antibody at 2 ug/ml. Green fluorescence highlights integrin alpha 3 expression, with prominent membranous and cytoplasmic staining. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes. This Integrin Alpha 3 Polyclonal Antibody detects CD49c, a cell adhesion receptor involved in extracellular matrix interactions and cellular migration.

ITGA3 Antibody by FACS in human U87 cells. Flow cytometry analysis of paraformaldehyde-fixed human U87 cells using ITGA3 Antibody at 1 ug per 1 x 10^6 cells. The overlay histogram shows a clear rightward fluorescence shift with ITGA3 staining (blue) compared with the rabbit IgG isotype control (green) and unstained control (red). Cells were labeled with Fluoro488-conjugated goat anti-rabbit IgG. This Integrin Alpha 3 Polyclonal Antibody detects CD49c in fixed U87 cells.