ITGA2B Antibody / Integrin Alpha 2b Antibody (RQ7859)

ITGA2B Antibody Staining in Human Spleen by IHC. Immunohistochemistry of FFPE human spleen tissue using ITGA2B Antibody shows distinct brown staining in scattered cells and cellular processes throughout the splenic tissue, against a predominantly hematoxylin-counterstained background. The distribution is consistent with detection of CD41-expressing elements within the spleen. Heat-induced epitope retrieval was performed by boiling the tissue sections in pH 8 EDTA for 20 min followed by cooling before antibody incubation. This staining demonstrates detection of integrin alpha 2b in human spleen tissue using this CD41 Antibody.

ITGA2B Antibody Detection in HEL and HeLa Human Cells by WB. Western blot testing of human HEL (lane 1) and HeLa (lane 2) cell lysates with ITGA2B Antibody shows a strong, discrete band in HEL cells migrating between the 95 and 130 kDa markers, with little to no corresponding signal visible in HeLa cells. The predicted molecular weight of integrin alpha 2b is approximately 113 kDa, while glycosylation can increase its apparent molecular weight. The differential signal between the two lysates demonstrates detection of the CD41 glycoprotein in HEL cells using this Integrin Alpha 2b Antibody.

ITGA2B Antibody Detection in HEL Human Erythroleukemia Cells by FACS. Flow cytometry analysis of human HEL erythroleukemia cells using ITGA2B Antibody at 1 ug/million cells shows a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat serum before antibody incubation. The strong separation of the blue population demonstrates detection of cell-surface integrin alpha 2b on HEL cells and supports use of this CD41 Antibody for examining CD41-positive cell populations by flow cytometry.

ITGA2B Antibody Staining in Rat Spleen by IHC. Immunohistochemistry of FFPE rat spleen tissue using ITGA2B Antibody shows distinct brown staining distributed among scattered cells and cellular structures throughout the splenic parenchyma. The signal is readily distinguishable from the blue hematoxylin-counterstained nuclei and demonstrates recognition of rat integrin alpha 2b. Heat-induced epitope retrieval was performed by boiling the tissue sections in pH 8 EDTA for 20 min followed by cooling before antibody incubation. This cross-species tissue staining further supports detection of the CD41 glycoprotein using this Integrin Alpha 2b Antibody.