Isocitrate Dehydrogenase Antibody 16H7 / IDH1 Protein Antibody [clone 16H7] (RQ6022)

Isocitrate Dehydrogenase Antibody 16H7 Staining of U-2 OS Osteosarcoma Cells IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells using Isocitrate Dehydrogenase Antibody, clone 16H7 (green), with DAPI nuclear counterstain (blue). Sections were heat treated by steaming in pH 6 citrate buffer for 20 min before staining. IDH1 Protein Antibody labeling is predominantly cytoplasmic, surrounding the DAPI-positive nuclei and consistent with the principal cellular localization of IDH1.

Isocitrate Dehydrogenase Antibody 16H7 Detects IDH1 in Human Cancer Cell Lines WB. Western blot analysis of human 1) HepG2, 2) Caco-2, 3) U-87 MG, 4) THP-1, 5) HeLa, 6) K562, 7) PC-3 and 8) HEK293 cell lysates using Isocitrate Dehydrogenase Antibody, clone 16H7. A prominent band is detected at approximately 46 kDa across all eight samples, consistent with the predicted molecular weight of IDH1. The relatively clean banding demonstrates recognition of endogenous IDH1 Protein across a diverse panel of human cell lines.

Isocitrate Dehydrogenase Antibody 16H7 Detects IDH1 in Rat and Mouse Samples WB. Western blot analysis of 1) rat liver tissue, 2) rat RH35 hepatoma cells and 3) mouse liver tissue using Isocitrate Dehydrogenase Antibody, clone 16H7. A strong, discrete band is detected at approximately 46 kDa in all three lanes, consistent with the predicted molecular weight of IDH1. The comparable target bands demonstrate recognition of endogenous IDH1 Protein in both rat and mouse samples with minimal additional reactivity.

Isocitrate Dehydrogenase Antibody 16H7 Detection in Caco-2 Colorectal Cancer Cells by FACS. Flow cytometry analysis of human Caco-2 colorectal adenocarcinoma cells using Isocitrate Dehydrogenase Antibody, clone 16H7. Cells were fixed with 4% paraformaldehyde, permeabilized, blocked with 10% normal goat serum, and incubated with antibody at 1 ug/million cells for 30 min at 20oC. DyLight 488-conjugated goat anti-mouse IgG was used as the secondary antibody. The antibody-stained population (blue) shows a clear rightward fluorescence shift relative to the mouse IgG isotype control (green) and unlabeled cells (red), demonstrating intracellular detection of IDH1 Protein.