Isocitrate Dehydrogenase Antibody 16H7 / IDH1 Protein Antibody [clone 16H7] (RQ6022)

Isocitrate Dehydrogenase Antibody 16H7 Detection in U-2 OS Human Osteosarcoma Cells IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells using Isocitrate Dehydrogenase Antibody, clone 16H7, shown in green with DAPI nuclear counterstain shown in blue. HIER was performed by steaming in pH 6 citrate buffer for 20 min. Green fluorescence is distributed predominantly throughout the cytoplasmic compartment, consistent with detection of IDH1 Protein in these human osteosarcoma cells.

Isocitrate Dehydrogenase Antibody 16H7 Detection Across Human Cell Lines WB. Western blot analysis using Isocitrate Dehydrogenase Antibody, clone 16H7, with lysates from 1) HepG2, 2) Caco-2, 3) U-87 MG, 4) THP-1, 5) HeLa, 6) K562, 7) PC-3 and 8) HEK293 cells. IDH1 has a predicted molecular weight of approximately 46 kDa. A prominent band is detected near the expected molecular weight across the eight human cell lines, supporting detection of IDH1 Protein in diverse cellular backgrounds.

Isocitrate Dehydrogenase Antibody 16H7 Detection in Rat and Mouse Samples WB. Western blot analysis using Isocitrate Dehydrogenase Antibody, clone 16H7, with lysates from 1) rat liver, 2) rat RH-35 cells and 3) mouse liver. IDH1 has a predicted molecular weight of approximately 46 kDa. A prominent band is detected near the expected molecular weight in each lane, demonstrating cross-species detection of IDH1 Protein in rat and mouse samples under the tested conditions.

Isocitrate Dehydrogenase Antibody 16H7 Detection in Caco-2 Human Colorectal Cancer Cells IF. Immunofluorescence staining of FFPE human Caco-2 colorectal adenocarcinoma cells using Isocitrate Dehydrogenase Antibody, clone 16H7, shown in green with DAPI nuclear counterstain shown in blue. HIER was performed by steaming in pH 6 citrate buffer for 20 min. Green fluorescence is visible predominantly throughout the cytoplasmic compartment surrounding the nuclei, consistent with detection of IDH1 Protein in these human colorectal cancer cells.