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Home >> Antibodies >> IRS1 Antibody / Insulin receptor substrate 1

IRS1 Antibody / Insulin receptor substrate 1 [clone 10I3] (RQ5507)

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  Catalog No Formulation Size Price (USD)  
Image RQ5507 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human placenta with IRS1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human placenta with IRS1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with IRS1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with IRS1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) T-47D, 3) Caco-2, 4) SW620, 5) HeLa and 6) Raji lysate with IRS1 antibody. Expected molecular weight: 132~185 kDa depending on phosphorylation level.
Flow cytometry testing of human PC-3 cells with IRS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRS1 antibody.
Flow cytometry testing of human U-2 OS cells with IRS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRS1 antibody.
IF/ICC staining of FFPE human MCF7 cells with IRS1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 10I3
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P35568
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence/Immunocytochemistry : 2-4ug/ml
Limitations This IRS1 antibody is available for research use only.
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Description

Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1(IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic(growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Application Notes

Optimal dilution of the IRS1 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids S1041-Q1242) was used as the immunogen for the IRS1 antibody.

Storage

After reconstitution, the IRS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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