IRS1 Antibody 10I3 / Insulin Receptor Substrate 1 Monoclonal Antibody [clone 10I3] (RQ5507)

IRS1 Antibody 10I3 Staining in Human Placenta by IHC. Immunohistochemistry staining of FFPE human placenta with IRS1 Antibody (clone 10I3) shows brown immunoreactivity within placental villi, with prominent staining in trophoblastic cells and weaker labeling in portions of the villous stroma. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10mM citrate buffer for 20 min followed by cooling before testing. The staining demonstrates detection of IRS1 in human placental tissue using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Staining in Human Lung Cancer by IHC. Immunohistochemistry staining of FFPE human lung cancer with IRS1 Antibody (clone 10I3) shows widespread brown immunoreactivity within the tumor-cell population. Labeling is predominantly cytoplasmic, with heterogeneous staining intensity across the tissue and comparatively little nuclear staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10mM citrate buffer for 20 min followed by cooling before testing. The cytoplasmic staining pattern demonstrates detection of IRS1 in human lung cancer using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Staining in Human Colon Cancer by IHC. Immunohistochemistry staining of FFPE human colon cancer with IRS1 Antibody (clone 10I3) shows brown immunoreactivity within malignant glandular epithelial cells. Staining is predominantly cytoplasmic and heterogeneous, with stronger labeling in portions of the tumor and comparatively weak staining in surrounding stromal areas. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10mM citrate buffer for 20 min followed by cooling before testing. The staining demonstrates detection of IRS1 in human colon cancer using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Detection in Multiple Human Cell Lines by WB. Western blot testing with IRS1 Antibody (clone 10I3) shows prominent high-molecular-weight bands in human A549 (lane 1), T-47D (lane 2), Caco-2 (lane 3), SW620 (lane 4), HeLa (lane 5), and Raji (lane 6) cell lysates. The major signals migrate at approximately 160-180 kDa, within the reported 132-185 kDa range for IRS1 depending on phosphorylation state. Signal intensity varies among the six samples, with particularly strong bands in T-47D, Caco-2, and HeLa lysates. This experiment demonstrates detection of IRS1 across multiple human cell lines using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Detection in Human PC-3 Prostate Cancer Cells by FACS. Flow cytometry analysis of human PC-3 prostate cancer cells with IRS1 Antibody (clone 10I3) at 1 ug/million cells shows a strong rightward fluorescence shift in the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat serum before antibody staining. The clear separation of the blue population from both controls demonstrates intracellular detection of IRS1 in PC-3 cells using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Detection in Human U-2 OS Osteosarcoma Cells by FACS. Flow cytometry analysis of human U-2 OS osteosarcoma cells with IRS1 Antibody (clone 10I3) at 1 ug/million cells shows a clear rightward fluorescence shift in the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat serum before antibody staining. The separation of the stained population from both controls demonstrates intracellular detection of IRS1 in U-2 OS cells using this Insulin Receptor Substrate 1 Monoclonal Antibody.

IRS1 Antibody 10I3 Detection in Human MCF7 Breast Cancer Cells by IF. Immunofluorescence staining of FFPE human MCF7 breast cancer cells with IRS1 Antibody (clone 10I3) at 2 ug/ml shows green IRS1-associated fluorescence distributed predominantly throughout the cytoplasm and around the blue DAPI-stained nuclei. Heat-induced epitope retrieval was performed by steaming the sample in pH 6 citrate buffer for 20 min. The cytoplasmic distribution is consistent with the role of IRS1 as an intracellular signaling adaptor and demonstrates cellular detection using this Insulin Receptor Substrate 1 Monoclonal Antibody.