IRF5 Antibody / Immune Response Regulator Antibody (R32219)

IRF5 Antibody Human, Mouse and Rat WB. Western blot analysis was performed using IRF5 Antibody. Lane 1: rat intestine lysate; lane 2: human HeLa cell lysate; lane 3: human COLO320 cell lysate; lane 4: mouse NIH3T3 cell lysate; lane 5: mouse HEPA1-6 cell lysate. A specific immunoreactive band is detected at approximately 57 kDa in all tested samples, consistent with the expected molecular weight of IRF5. These results demonstrate that this Immune Response Regulator Antibody is suitable for Western blot detection of IRF5 across human, mouse, and rat samples.

IRF5 Antibody Human Breast Cancer IHC. Immunohistochemistry was performed on FFPE human breast cancer tissue using IRF5 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Predominantly nuclear staining is observed in the malignant epithelial cells, consistent with the role of IRF5 as an immune response-regulating transcription factor. These results demonstrate that this Immune Response Regulator Antibody is suitable for immunohistochemical detection of IRF5 in formalin-fixed, paraffin-embedded human breast cancer tissue.

IRF5 Antibody Mouse Spleen IHC. Immunohistochemistry was performed on FFPE mouse spleen tissue using IRF5 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Distinct nuclear staining is observed within splenic immune cell populations, consistent with the role of IRF5 as an immune response-regulating transcription factor. These results demonstrate that this Immune Response Regulator Antibody is suitable for immunohistochemical detection of IRF5 in formalin-fixed, paraffin-embedded mouse spleen tissue.

IRF5 Antibody Rat Spleen IHC. Immunohistochemistry was performed on FFPE rat spleen tissue using IRF5 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Predominantly nuclear staining is observed throughout splenic immune cell populations, consistent with the role of IRF5 as an immune response-regulating transcription factor. These results demonstrate that this Immune Response Regulator Antibody is suitable for immunohistochemical detection of IRF5 in formalin-fixed, paraffin-embedded rat spleen tissue.