MUM1 Antibody / Multiple Myeloma Oncogene 1 Antibody (R31635)

MUM1 Antibody Detection in Human HeLa and Jurkat Cells by WB. Western blot testing of human HeLa (lane 1) and Jurkat T-cell (lane 2) lysates with MUM1 Antibody shows strong, discrete bands migrating at approximately 40 kDa in both samples. This is below the predicted molecular weight of approximately 51 kDa for full-length IRF4. Because the reason for this migration difference is not established by the experiment, I would retain the observed molecular weight without assigning it to a specific processing or modification mechanism. The consistent bands in both lysates demonstrate antibody reactivity using this Multiple Myeloma Oncogene 1 Antibody.

MUM1 Antibody Detection of Recombinant Human IRF4 Protein by WB. Western blot testing of 0.5 ng recombinant human IRF4 protein with MUM1 Antibody shows a single strong, discrete band migrating at approximately 38-40 kDa. The isolated signal demonstrates recognition of the recombinant IRF4 antigen by this Multiple Myeloma Oncogene 1 Antibody. Because the recombinant protein construct and its expected molecular weight are not specified on the product page, the observed migration should not be compared directly with the approximately 51 kDa predicted molecular weight of full-length endogenous IRF4.

MUM1 Antibody Staining in Human Tonsil by IHC. Immunohistochemistry staining of FFPE human tonsil tissue with MUM1 Antibody shows strong brown nuclear labeling in a distinct population of lymphoid cells, with numerous surrounding nuclei remaining negative. The discrete nuclear staining pattern is consistent with the localization of MUM1/IRF4 as a transcription factor and highlights the heterogeneous expression expected among lymphoid cell populations. The staining demonstrates detection of IRF4 in human tonsil tissue using this Multiple Myeloma Oncogene 1 Antibody.