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Home >> Antibodies >> Irf3 Antibody / Interferon regulatory factor 3

Irf3 Antibody / Interferon regulatory factor 3 [clone 7B10] (RQ6375)

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  Catalog No Formulation Size Price (USD)  
Image RQ6375 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of mouse HEPA1-6 cells with Irf3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Irf3 antibody.
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Flow cytometry testing of mouse HEPA1-6 cells with Irf3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Irf3 antibody.
Western blot testing of 1) rat brain, 2) mouse lung, 3) mouse brain and 4) mouse NIH 3T3 cell lysate with Irf3 antibody. Predicted molecular weight ~47 kDa.
Immunofluorescent staining of FFPE mouse RM-1 cells with Irf3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 7B10
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P70671
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Irf3 antibody is available for research use only.
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Description

IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Application Notes

Optimal dilution of the Irf3 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids M1-I419) was used as the immunogen for the Irf3 antibody.

Storage

After reconstitution, the Irf3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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