IRF3 Antibody / Innate Immune Signaling Antibody [clone PCRP-IRF3-6C8] (V9201)

IRF3 Antibody MCF-7 Immunofluorescence. Immunofluorescent analysis of human MCF-7 cells stained with IRF3 Antibody demonstrates predominantly cytoplasmic and perinuclear green fluorescence consistent with IRF3-associated innate immune signaling and interferon regulatory pathway localization. This innate immune signaling antibody highlights antiviral response-associated transcriptional regulatory biology and stress-response signaling organization in epithelial-derived tumor cells. Phalloidin co-staining (red) labels actin-associated cytoskeletal structures.

IRF3 Antibody K562 Immunofluorescence. Immunofluorescent analysis of human K562 cells stained with IRF3 Antibody demonstrates prominent punctate cytoplasmic and perinuclear green fluorescence consistent with IRF3-associated innate immune signaling and interferon regulatory pathway localization. This innate immune signaling antibody highlights antiviral response-associated transcriptional regulatory biology and stress-response signaling organization in hematologic-derived cells. Phalloidin co-staining (red) labels actin-associated cytoskeletal structures.

IRF3 Antibody MCF-7 FACS. Flow cytometry analysis of PFA-fixed human MCF-7 cells stained with IRF3 Antibody demonstrates a distinct right-shifted fluorescence population relative to unstained cells, consistent with IRF3-associated innate immune signaling and interferon regulatory pathway expression. This innate immune signaling antibody supports characterization of antiviral response-associated transcriptional regulatory proteins in epithelial-derived tumor cells. Blue=IRF3 antibody, Red=unstained cells.

IRF3 Antibody K562 FACS. Flow cytometry analysis of PFA-fixed human K562 cells stained with IRF3 Antibody demonstrates a distinct right-shifted fluorescence population relative to unstained cells, consistent with IRF3-associated innate immune signaling and interferon regulatory pathway expression. This innate immune signaling antibody supports characterization of antiviral response-associated transcriptional regulatory proteins in hematologic-derived cells. Blue=IRF3 antibody, Red=unstained cells.

IRF3 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with IRF3 Antibody demonstrates a distinct right-shifted fluorescence population relative to unstained cells, consistent with IRF3-associated innate immune signaling and interferon regulatory pathway expression. This innate immune signaling antibody supports characterization of antiviral response-associated transcriptional regulatory proteins in epithelial-derived cells. Blue=IRF3 antibody, Red=unstained cells.

IRF3 Antibody U87 FACS. Flow cytometry analysis of PFA-fixed human U87 cells stained with IRF3 Antibody demonstrates a distinct right-shifted fluorescence population relative to unstained cells, consistent with IRF3-associated innate immune signaling and interferon regulatory pathway expression. This innate immune signaling antibody supports characterization of antiviral response-associated transcriptional regulatory proteins in glioblastoma-derived cells. Blue=IRF3 antibody, Red=unstained cells.

IRF3 Antibody Raji FACS. Flow cytometry analysis of PFA-fixed human Raji cells stained with IRF3 Antibody demonstrates a distinct right-shifted fluorescence population relative to unstained cells, consistent with IRF3-associated innate immune signaling and interferon regulatory pathway expression. This innate immune signaling antibody supports characterization of antiviral response-associated transcriptional regulatory proteins in B lymphocyte-derived cells. Blue=IRF3 antibody, Red=unstained cells.

IRF3 Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using IRF3 Antibody detects a prominent band at approximately 47-50 kDa, consistent with the predicted molecular weight of Interferon regulatory factor 3 / IRF3. This innate immune signaling antibody highlights IRF3-associated antiviral response pathways and interferon-regulatory transcriptional signaling in epithelial-derived cells.

IRF3 Antibody MCF7 WB. Western blot analysis of human MCF7 cell lysate using IRF3 Antibody detects a strong band at approximately 47-50 kDa, consistent with the predicted molecular weight of Interferon regulatory factor 3 / IRF3. This innate immune signaling antibody highlights IRF3-associated antiviral response pathways and interferon-regulatory transcriptional signaling in epithelial-derived breast cancer cells.

IRF3 Antibody HuProt Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF3 Antibody demonstrates highly selective recognition of IRF3 with exceptionally strong separation from non-target proteins across the tested human proteome. These results demonstrate the foremost specificity of the PCRP-IRF3-6C8 mAb and support highly specific detection of innate immune-associated signaling pathways and interferon regulatory proteins. The Z-score represents the strength of antibody binding signal relative to the overall array mean, while the S-score reflects target specificity relative to the next highest ranked protein signal. The markedly elevated S-score separation observed for IRF3 supports preferential target recognition with minimal off-target binding across the tested human proteome.

SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-6C8) as confirmation of integrity and purity.