IRF3 Antibody / Innate Immune Transcription Factor Antibody (FY13467)

IRF3 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemical staining of FFPE human colorectal adenocarcinoma tissue with IRF3 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. IRF3 immunoreactivity is observed predominantly within the cytoplasm of malignant epithelial cells, consistent with the expected localization of this innate immune transcription factor, which becomes activated and translocates to the nucleus following phosphorylation to regulate type I interferon production and antiviral gene expression. Cell nuclei are counterstained with hematoxylin (blue).

IRF3 Antibody Human Bladder Cancer IHC. Immunohistochemical staining of FFPE human bladder urothelial carcinoma tissue with IRF3 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. IRF3 immunoreactivity is observed predominantly within the cytoplasm of malignant urothelial cells, consistent with the expected localization of this innate immune transcription factor, which undergoes phosphorylation-dependent nuclear translocation to activate type I interferon signaling and antiviral gene expression. Cell nuclei are counterstained with hematoxylin (blue).

IRF3 Antibody Human Cell Line WB. Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Raji, and 4) human HepG2 cell lysates with IRF3 antibody at 0.5 ug/ml. A prominent immunoreactive band is detected at approximately 50-55 kDa in all four human cell lines, consistent with the expected migration of IRF3 despite its predicted molecular weight of approximately 47 kDa. The results demonstrate specific detection of this innate immune transcription factor, which regulates type I interferon production and antiviral gene expression following activation by pattern recognition receptor signaling pathways.