IQCE Antibody / IQ domain-containing protein E (FY12471)

Immunofluorescent staining of IQCE using anti-IQCE antibody (green) and anti-Beta Tubulin antibody (red). IQCE was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-IQCE antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Donkey Anti-Rabbit IgG and DyLight 594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of IQCE using anti-IQCE antibody. Lane 1: human MCF-7 whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQCE antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of IQCE is ~77 kDa.