Involucrin Antibody SPM259 / Mouse Monoclonal [clone SPM259] (V9036)

Description

Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

For an alternative recombinant monoclonal targeting this protein, see our Involucrin Antibody IVRN/2113R / IVL Monoclonal Antibody page.

Application Notes

The optimal dilution of the Involucrin Antibody SPM259 for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin Antibody SPM259. Its epitope maps between amino acids 421-568 of human involucrin.

Storage

Store the Involucrin Antibody SPM259 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).