Inverted Formin 2 Antibody / Formin Protein Antibody (FY13470)

Inverted Formin 2 Antibody Human, Rat and Mouse WB. Western blot testing of human A431, HeLa, Caco-2 and A549 cell lysates, rat C6 cell lysate and mouse NIH/3T3 cell lysate with Inverted Formin 2 Antibody at 0.5 ug/ml. Lane 1: human A431; Lane 2: human HeLa; Lane 3: human Caco-2; Lane 4: human A549; Lane 5: rat C6; Lane 6: mouse NIH/3T3. Predicted molecular weight: ~136 kDa; the INF2 protein is routinely observed at approximately 170-180 kDa due to isoform variation, protein structure and migration characteristics during SDS-PAGE. Inverted Formin 2 Antibody / Formin Protein Antibody detects endogenous INF2 expression and supports studies of actin cytoskeleton remodeling, intracellular organization and formin protein regulation pathways.

Inverted Formin 2 Antibody Human Stomach Cancer IHC. Immunohistochemistry staining of FFPE human stomach cancer tissue with Inverted Formin 2 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, intracellular organization and cell structure regulation pathways.

Inverted Formin 2 Antibody Human Colon Cancer IHC. Immunohistochemistry staining of FFPE human colon cancer tissue with Inverted Formin 2 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, intracellular organization and formin-mediated cellular regulation pathways.

Inverted Formin 2 Antibody Human Thyroid Cancer IHC. Immunohistochemistry staining of FFPE human thyroid cancer tissue with Inverted Formin 2 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, intracellular organization and formin-mediated cellular regulation pathways.

Inverted Formin 2 Antibody Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Inverted Formin 2 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, neuronal cell structure and intracellular organization pathways.

Inverted Formin 2 Antibody Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Inverted Formin 2 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, neuronal cell biology and formin-mediated cellular regulation pathways.

Inverted Formin 2 Antibody A549 Cell IF. Immunofluorescent staining of human A549 cells with Inverted Formin 2 Antibody at 5 ug/ml. Enzyme antigen retrieval was performed for 15 minutes, followed by blocking with 10% goat serum. Cells were incubated with rabbit anti-INF2 antibody overnight at 4°C, followed by DyLight 488 conjugated goat anti-rabbit IgG secondary antibody (green). Nuclei were counterstained with DAPI (blue). Inverted Formin 2 Antibody / Formin Protein Antibody demonstrates cytoplasmic staining consistent with INF2 expression and supports studies of actin cytoskeleton remodeling, intracellular organization and formin-mediated cellular regulation pathways.

Inverted Formin 2 Antibody Human Cell IP. Immunoprecipitation of INF2 protein from 500 ug of human HeLa whole cell lysate using 2 ug of Inverted Formin 2 Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and rabbit IgG control samples included for comparison. INF2 was detected at approximately 180 kDa, above the predicted 136 kDa molecular weight due to isoform variation, protein structure and SDS-PAGE migration characteristics. Inverted Formin 2 Antibody / Formin Protein Antibody successfully enriches endogenous INF2 protein and supports studies of actin cytoskeleton remodeling, protein interactions and formin-mediated cellular regulation pathways.