Intestinal FABP Antibody / Intestinal fatty acid-binding protein [clone FABP2/6345] (V5887)

Western blot analysis of FABP2 in intestinal tissues. Western blot analysis was performed using tissue lysates from human intestine, mouse intestine, rat intestine, hamster intestine, guinea pig intestine, and rabbit intestine probed with Intestinal FABP/Intestinal fatty acid-binding protein antibody (clone FABP2/6345). A distinct immunoreactive band is observed at the predicted molecular weight of FABP2 across all intestinal samples, consistent with conserved expression of Intestinal FABP in absorptive intestinal epithelium. Predicted molecular weight ~15 kDa.

Analysis of Protein Array containing more than 19,000 full-length human proteins using Intestinal FABP/Intestinal fatty acid-binding protein antibody (clone FABP2/6345). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human kidney tissue stained with Intestinal FABP/Intestinal fatty acid-binding protein antibody (clone FABP2/6345). Brown chromogenic signal indicates FABP2-positive epithelial cells, while surrounding stromal cells show minimal staining. Inset shows PBS in place of the primary antibody as a secondary-only negative control. Antigen retrieval was performed by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes.

Western blot analysis of human small intestine tissue lysate using Intestinal FABP/Intestinal fatty acid-binding protein antibody (FABP2/6345).

Western blot analysis of Human Intestine, Mouse Intestine and Rat Intestinal FABP/Intestinal fatty acid-binding protein antibody (FABP2/6345).

SDS-PAGE Analysis of purified Intestinal FABP/Intestinal fatty acid-binding protein antibody (FABP2/6345). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human small intestine tissue lysate using Intestinal FABP/Intestinal fatty acid-binding protein antibody (FABP2/6345).