- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Intersectin 1 Antibody / ITSN1 (RQ8715)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ8715 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q15811 |
| Localization | Cell membrane, cytoplasm |
| Applications | Western Blot : 1-2ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This Intersectin 1 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Intersectin 1 antibody is an essential reagent for research on endocytosis, intracellular trafficking, and signal transduction. The encoded protein, ITSN1, is a multidomain scaffold that links clathrin-mediated vesicle formation with cytoskeletal regulation and small GTPase signaling. Intersectin 1 contains EH domains, SH3 domains, and a Dbl homology domain that allow it to interact with clathrin coat components, actin regulators, and endocytic enzymes. This architecture enables ITSN1 to integrate membrane dynamics with signaling pathways, making it a central coordinator of cellular communication.
In clathrin-mediated endocytosis, intersectin 1 organizes protein complexes required for receptor uptake and vesicle recycling. It binds to proteins such as dynamin and synaptojanin to support vesicle scission, while also engaging actin modulators to regulate cytoskeletal rearrangements during endocytic pit formation. In neurons, ITSN1 is enriched at synapses, where it contributes to efficient synaptic vesicle turnover and influences neurotransmitter release. This activity underscores its importance for neuronal signaling and synaptic plasticity.
Beyond its role in trafficking, ITSN1 regulates Rho family GTPases through its Dbl homology domain, which acts as a guanine nucleotide exchange factor (GEF) for Cdc42. By activating Cdc42, intersectin 1 drives actin cytoskeleton remodeling, cell polarity, and migration. This dual function in both endocytosis and GTPase signaling highlights ITSN1 as a multifunctional hub linking membrane dynamics with cellular responses to environmental stimuli.
Dysregulation of intersectin 1 has been associated with human disease. Genetic alterations in ITSN1 are implicated in Down syndrome, where dosage effects contribute to neurodevelopmental abnormalities. Changes in ITSN1 expression have also been observed in Alzheimer disease, suggesting a role in synaptic dysfunction and degeneration. In cancer research, ITSN1 is studied for its ability to influence receptor endocytosis and downstream survival pathways, making it a potential biomarker or therapeutic target.
The Intersectin 1 antibody is widely applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect protein expression and localization across tissues. These applications support studies of endocytosis, neuronal biology, and signaling in normal and disease states. For researchers examining vesicle trafficking, actin regulation, or developmental disorders, the Intersectin 1 antibody provides a reliable detection tool. NSJ Bioreagents supplies validated antibodies designed to deliver reproducibility and accuracy for advanced molecular studies.
Optimal dilution of the Intersectin 1 antibody should be determined by the researcher.
An E.coli-derived human recombinant protein (amino acids A522-P1721) was used as the immunogen for the Intersectin 1 antibody.
After reconstitution, the Intersectin 1 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
