Interleukin-6 Antibody / IL6 Antibody [clone IL6/4647] (V4027)

Interleukin-6 Protein Microarray Specificity Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Interleukin-6 Antibody / IL6 Antibody (clone IL6/4647) demonstrates exceptional target specificity for IL6. The intended target ranked first among all proteins evaluated, generating a Z-score of 116.69. The next highest ranked proteins, SMU1 and RGS20, produced substantially lower signals, resulting in an S-score of 105.30 for IL6. Because an S-score above 2.5 is generally considered indicative of target specificity, these results strongly support selective recognition of Interleukin 6 by the IL6/4647 monoclonal antibody. The large separation between IL6 and all other detected proteins demonstrates the foremost specificity of the IL6/4647 mAb and supports its application in studies of cytokine signaling, inflammatory responses, immune regulation, and disease-associated inflammatory pathways.

Interleukin-6 Antibody Human Adrenal Gland IHC. Immunohistochemical staining of FFPE human adrenal gland tissue using Interleukin-6 Antibody / IL6 Antibody (clone IL6/4647) demonstrates granular cytoplasmic HRP-DAB brown staining within scattered adrenal cortical cells. The observed staining pattern is consistent with expression of IL6, a multifunctional cytokine involved in inflammatory signaling, immune regulation, stress responses, and communication between endocrine and immune systems. Detection of IL6 within adrenal tissue supports the utility of this antibody for investigating cytokine-mediated signaling pathways and inflammatory mechanisms across endocrine and immune-related research applications. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.