Interleukin 5 Antibody IL5/4161 T Cell-Replacing Factor V9534

	Catalog No	Formulation	Size	Price (USD)
	V9534-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9534-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9534SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

Interleukin 5 Antibody IL5/4161 Protein Microarray Validated. Protein microarray analysis of Interleukin 5 Antibody clone IL5/4161 on a HuProt array containing more than 19,000 full-length human proteins shows IL5 as the dominant target signal, with strong separation from the remaining proteins on the array. The Z-score represents the number of standard deviations a protein signal lies above the array mean, while the S-score measures the separation between the intended target and the next-ranked protein. The pronounced IL5 signal and target separation support the specificity of this IL-5 Antibody.

Interleukin 5 Antibody IL5/4161 Staining in Human Adrenal Gland IHC. Immunohistochemistry of FFPE human adrenal gland tissue using Interleukin 5 Antibody clone IL5/4161 shows widespread brown staining with heterogeneous intensity among cells in the tissue. Sections underwent HIER by boiling in pH 9 10 mM Tris containing 1 mM EDTA for 20 min followed by cooling before testing. The staining demonstrates detection of IL-5 protein in human adrenal gland tissue.

Interleukin 5 Antibody IL5/4161 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2 ug of purified, BSA-free Interleukin 5 Antibody clone IL5/4161 under non-reducing (NR) and reducing (R) conditions shows the expected immunoglobulin migration pattern. The intact monoclonal antibody migrates near 150 kDa under non-reducing conditions, while reduction produces prominent heavy and light chain bands at approximately 50 kDa and 25 kDa, respectively. The defined banding pattern confirms the integrity of the purified IL-5 Antibody preparation.

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Interleukin 5 Antibody IL5/4161 Protein Microarray Validated. Protein microarray analysis of Interleukin 5 Antibody clone IL5/4161 on a HuProt array containing more than 19,000 full-length human proteins shows IL5 as the dominant target signal, with strong separation from the remaining proteins on the array. The Z-score represents the number of standard deviations a protein signal lies above the array mean, while the S-score measures the separation between the intended target and the next-ranked protein. The pronounced IL5 signal and target separation support the specificity of this IL-5 Antibody.

Interleukin 5 Antibody IL5/4161 Staining in Human Adrenal Gland IHC. Immunohistochemistry of FFPE human adrenal gland tissue using Interleukin 5 Antibody clone IL5/4161 shows widespread brown staining with heterogeneous intensity among cells in the tissue. Sections underwent HIER by boiling in pH 9 10 mM Tris containing 1 mM EDTA for 20 min followed by cooling before testing. The staining demonstrates detection of IL-5 protein in human adrenal gland tissue.

Interleukin 5 Antibody IL5/4161 Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2 ug of purified, BSA-free Interleukin 5 Antibody clone IL5/4161 under non-reducing (NR) and reducing (R) conditions shows the expected immunoglobulin migration pattern. The intact monoclonal antibody migrates near 150 kDa under non-reducing conditions, while reduction produces prominent heavy and light chain bands at approximately 50 kDa and 25 kDa, respectively. The defined banding pattern confirms the integrity of the purified IL-5 Antibody preparation.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	IL5/4161
Purity	Protein A/G affinity
UniProt	P05113
Localization	Secreted, Extracellular
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This Interleukin 5 Antibody IL5/4161 / IL-5 Antibody is available for research use only.
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Description

Interleukin 5 Antibody IL5/4161 recognizes interleukin 5 (IL-5), a secreted cytokine encoded by the IL5 gene that plays an important role in eosinophil development, activation, and survival. IL-5 is strongly associated with type 2 immune responses and is produced by several immune-cell populations, including activated T helper 2 cells and group 2 innate lymphoid cells. The cytokine was historically described as T cell-replacing factor because of its ability to promote B-cell differentiation and antibody production.

IL-5 exerts its biological effects through a heterodimeric receptor composed of the ligand-binding IL5RA alpha subunit and the common beta subunit CSF2RB (CD131). CSF2RB is also shared by the receptor systems for IL-3 and GM-CSF. Binding of IL-5 to its receptor activates intracellular signaling pathways including JAK-STAT, PI3K-AKT, and MAPKs, connecting cytokine recognition with cellular survival, proliferation, differentiation, and effector functions.

Eosinophils are particularly responsive to IL-5, which promotes their differentiation from hematopoietic progenitors and supports the survival and activation of mature cells. IL-5 is consequently an important subject in research involving eosinophilic inflammation, asthma, allergic disease, and host responses to parasitic infection. Its effects on B-cell biology and antibody production also connect IL-5 signaling with broader mechanisms of adaptive and humoral immunity.

Clone IL5/4161 is a mouse monoclonal antibody generated against recombinant mature human IL5 protein. The antibody has also been evaluated on a HuProt protein microarray containing more than 19,000 full-length human proteins to assess target specificity. As an IL-5 Antibody, clone IL5/4161 provides a defined reagent for investigating this type 2 cytokine and its relationship to eosinophil biology and immune regulation. NSJ Bioreagents supplies IL5/4161 for research use only.

An Interleukin 5 Antibody can be used to investigate IL-5 expression and molecular mechanisms connecting cytokine signaling, eosinophil biology, humoral immunity, and type 2 inflammatory responses.

Explore our Immunology Antibodies page for additional antibodies targeting cytokines and regulators of immune-cell development, activation, and inflammatory signaling.

Application Notes

Optimal dilution of the Interleukin 5 Antibody IL5/4161 / IL-5 Antibodyy should be determined by the researcher.

Immunogen

A recombinant fragment of human IL5 protein corresponding to the mature protein sequence was used as the immunogen for the Interleukin 5 Antibody IL5/4161.

Storage

Aliquot the Interleukin 5 Antibody IL5/4161 and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

IL5 antibody, IL-5 antibody, interleukin 5 antibody, interleukin-5 antibody, T cell-replacing factor antibody, T-cell replacing factor antibody, TRF antibody

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Interleukin 5 Antibody IL5/4161 / IL-5 Antibody [clone IL5/4161] (V9534)

Description

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Immunogen

Storage

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Area of Research

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Protocols

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NSJ Bioreagents