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Home >> Antibodies >> Interleukin 10 Antibody / IL-10

Interleukin 10 Antibody / IL-10 (RQ7264)

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  Catalog No Formulation Size Price (USD)  
Image RQ7264 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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IHC staining of FFPE human tonsil tissue with Interleukin 10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P22301
Localization Cell surface, cytoplasmic, secreted
Applications Immunohistochemistry (FFPE) : 2-5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Interleukin 10 antibody is available for research use only.
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Description

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-g, IL-2, IL-3, TNFa and GM-CSF made by cells such as macrophages and regulatory T-cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

For a recombinant rabbit monoclonal with complementary IL-10 validation, see our IL-10 Antibody IL10/2651R / CSIF Antibody page.

Application Notes

Optimal dilution of the Interleukin 10 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K137-N178) was used as the immunogen for the Interleukin 10 antibody.

Storage

After reconstitution, the Interleukin 10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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