IRF7 Antibody / Antiviral Response Protein Antibody (RQ7267)

IRF7 Antibody Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue using IRF7 Antibody / Antiviral Response Protein Antibody demonstrates widespread nuclear and cytoplasmic HRP-DAB brown staining throughout splenic lymphoid cell populations. Strong immunoreactivity is observed within cells populating the white pulp, consistent with expression of Interferon Regulatory Factor 7 (IRF7), a transcription factor that serves as a master regulator of type I interferon production and antiviral immune responses. The staining pattern aligns with the established role of IRF7 in innate immune activation, host defense signaling, and regulation of interferon-dependent gene expression within lymphoid tissues. HIER: boil tissue sections in pH 8 EDTA for 20 minutes and allow to cool before testing.

IRF7 Antibody Human 293T Cell WB. Western blot analysis of human 293T cell lysate using IRF7 Antibody / Antiviral Response Protein Antibody demonstrates a prominent immunoreactive band at approximately 65-70 kDa. The observed molecular weight is higher than the predicted size of Interferon Regulatory Factor 7 (IRF7) (~54 kDa), which is consistent with phosphorylation and other post-translational modifications known to regulate IRF7 activation, dimerization, and nuclear translocation during antiviral signaling. IRF7 functions as a master regulator of type I interferon production and innate immune responses, controlling expression of genes involved in host defense against viral infection. The strong band observed in 293T cells is consistent with expression of IRF7 and its modified forms associated with immune signaling pathways.

IRF7 Antibody Human JK Cell FACS. Flow cytometry analysis of human JK cells using IRF7 Antibody / Antiviral Response Protein Antibody demonstrates a clear rightward shift of the positive cell population (blue) relative to the isotype control (green) and unstained cells (red), consistent with specific detection of Interferon Regulatory Factor 7 (IRF7). IRF7 is a transcription factor that functions as a master regulator of type I interferon production and antiviral immune responses. The observed staining profile supports expression of IRF7 in JK cells and is consistent with its established role in innate immune signaling, interferon pathway activation, and host defense mechanisms. Cells were stained using 1 µg antibody per million cells following blocking with goat serum. Red = cells alone; Green = isotype control; Blue = IRF7 antibody.