Interferon Regulatory Factor 5 Antibody / IRF5 Antibody (RQ8687)

Interferon Regulatory Factor 5 Antibody A549 Cell IF. Immunofluorescence staining was performed on FFPE human A549 cells using Interferon Regulatory Factor 5 Antibody (red). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Phalloidin-AF488 (green) was used to visualize the actin cytoskeleton. Distinct nuclear staining of IRF5 is observed with minimal background, consistent with the function of IRF5 as a transcription factor. These results demonstrate that this IRF5 Antibody is suitable for immunofluorescent detection of Interferon Regulatory Factor 5 in formalin-fixed, paraffin-embedded human cells.

Interferon Regulatory Factor 5 Antibody Human, Rat and Mouse Cell WB. Western blot analysis was performed using Interferon Regulatory Factor 5 Antibody. Lane 1: human HeLa cell lysate; lane 2: human A549 cell lysate; lane 3: human SH-SY5Y cell lysate; lane 4: rat PC-12 cell lysate; lane 5: rat C6 cell lysate; lane 6: mouse RAW264.7 cell lysate; lane 7: mouse NIH 3T3 cell lysate. A specific immunoreactive band is detected at approximately 57 kDa in all tested samples, consistent with the predicted molecular weight of IRF5. These results demonstrate that this IRF5 Antibody is suitable for Western blot detection of Interferon Regulatory Factor 5 across human, rat, and mouse cell lines.

Interferon Regulatory Factor 5 Antibody SH-SY5Y Cell FACS. Flow cytometric analysis was performed on fixed and permeabilized human SH-SY5Y cells using Interferon Regulatory Factor 5 Antibody at 1 ug/10^6 cells following blocking with normal goat serum. The red histogram represents unstained cells, the green histogram represents the isotype control, and the blue histogram represents cells stained with the Interferon Regulatory Factor 5 antibody. A clear rightward shift of the antibody-stained population relative to both control populations demonstrates specific intracellular binding and confirms IRF5 expression in SH-SY5Y cells. These results demonstrate that this IRF5 Antibody is suitable for flow cytometric detection of Interferon Regulatory Factor 5.