Interferon Regulatory Factor 4 Antibody / IRF4 Polyclonal Antibody (RQ8689)

Interferon Regulatory Factor 4 Antibody Detection in Human A549 Lung Cancer Cells by IF. Immunofluorescence staining of FFPE human A549 lung cancer cells with Interferon Regulatory Factor 4 Antibody shows red IRF4-associated fluorescence concentrated predominantly within the nuclear and perinuclear regions, while Phalloidin-iFluor 488 highlights the actin cytoskeleton in green. Heat-induced epitope retrieval was performed by steaming the sample in pH 6 citrate buffer for 20 min. The concentrated intracellular red signal is consistent with the nuclear localization of this transcription factor and demonstrates cellular detection of IRF4 using this IRF4 Polyclonal Antibody.

Interferon Regulatory Factor 4 Antibody Detection in Human Hut-78 T Cells by WB. Western blot testing of human Hut-78 T-cell lysate with Interferon Regulatory Factor 4 Antibody shows a strong, discrete band migrating just above the 50 kDa molecular weight marker, closely corresponding to the predicted molecular weight of approximately 51 kDa for human IRF4. Little additional reactivity is visible across the displayed molecular weight range, producing a clean target-region signal. The well-defined band demonstrates detection of endogenous IRF4 in human Hut-78 cells using this IRF4 Polyclonal Antibody.