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Home >> Antibodies >> Interferon regulatory factor 3 Antibody / Irf3

Interferon regulatory factor 3 Antibody / Irf3 [clone 3B4] (RQ6374)

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  Catalog No Formulation Size Price (USD)  
Image RQ6374 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain, 2) rat C6, 3) mouse lung, 4) mouse brain and 5) mouse NIH 3T3 cell lysate with Interferon regulatory factor 3 antibody. Predicted molecular weight ~47 kDa.
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Western blot testing of 1) rat brain, 2) rat C6, 3) mouse lung, 4) mouse brain and 5) mouse NIH 3T3 cell lysate with Interferon regulatory factor 3 antibody. Predicted molecular weight ~47 kDa.
Immunofluorescent staining of FFPE mouse RM-1 cells with Interferon regulatory factor 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of mouse HEPA1-6 cells with Interferon regulatory factor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Interferon regulatory factor 3 antibody.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 3B4
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P70671
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Interferon regulatory factor 3 antibody is available for research use only.
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Description

IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Application Notes

Optimal dilution of the Interferon regulatory factor 3 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids M1-I419) was used as the immunogen for the Interferon regulatory factor 3 antibody.

Storage

After reconstitution, the Interferon regulatory factor 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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