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Home >> Antibodies >> Interferon alpha receptor 1 Antibody / IFNAR1

Interferon alpha receptor 1 Antibody / IFNAR1 (F54976)

  Catalog No Formulation Size Price (USD)  
Image F54976-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54976-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with Interferon alpha receptor 1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat, 4) brain, 5) heart and 6) K562 cell lysate with Interferon alpha receptor 1 antibody. Expected molecular weight: 64-135 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human K562 cells with Interferon alpha receptor 1 antibody; Blue=isotype control, Green= Interferon alpha receptor 1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt P17181
Localization Cytoplasmic, cell membrane
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This Interferon alpha receptor 1 antibody is available for research use only.
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Description

IFNAR1 is the receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha-and beta-subunits themselves.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Interferon alpha receptor 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 162-188 from the human protein was used as the immunogen for the Interferon alpha receptor 1 antibody.

Storage

Aliquot the Interferon alpha receptor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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