Intercellular Adhesion Molecule 1 Antibody 6F2C3 / LFA-1 Ligand Antibody [clone 6F2C3] (RQ7002)

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Human Rectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human moderately differentiated rectal adenocarcinoma tissue with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 shows prominent membranous and cell-associated staining throughout the tumor tissue. This LFA-1 Ligand Antibody was tested following heat-induced epitope retrieval by boiling tissue sections in pH 8 EDTA for 20 min and allowing them to cool before testing.

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Human Renal Carcinoma IHC. Immunohistochemistry staining of FFPE human renal carcinoma tissue with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 shows heterogeneous staining, with membranous and cell-associated immunoreactivity in subsets of tumor and stromal cells. This LFA-1 Ligand Antibody was tested following heat-induced epitope retrieval by boiling tissue sections in pH 8 EDTA for 20 min and allowing them to cool before testing.

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Human Ovarian Serous Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human ovarian serous adenocarcinoma tissue with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 shows distinct membranous staining along epithelial tumor structures, with comparatively weaker staining in surrounding areas. This LFA-1 Ligand Antibody was tested following heat-induced epitope retrieval by boiling tissue sections in pH 8 EDTA for 20 min and allowing them to cool before testing.

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 shows strong staining in regions surrounding lymphoid follicles, with lower immunoreactivity within the more densely cellular follicular compartment. This LFA-1 Ligand Antibody was tested following heat-induced epitope retrieval by boiling tissue sections in pH 8 EDTA for 20 min and allowing them to cool before testing.

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Human Cell Lines WB. Western blot testing of human Raji, HeLa, K562, and HepG2 cell lysates with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 shows predominant bands at approximately 85-100 kDa, with the strongest signal in HepG2 cells. Lanes 1-4: Raji, HeLa, K562, and HepG2, respectively. Although unmodified ICAM1 has a predicted molecular weight of approximately 58 kDa, extensive N-linked glycosylation produces mature forms in the 75-115 kDa range, consistent with the bands detected by this LFA-1 Ligand Antibody.

Intercellular Adhesion Molecule 1 Antibody 6F2C3 in Caco-2 Colorectal Adenocarcinoma Cells FACS. Flow cytometry testing of human Caco-2 colorectal adenocarcinoma cells with Intercellular Adhesion Molecule 1 Antibody clone 6F2C3 at 1 ug/million cells shows a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red), demonstrating detectable cell-associated ICAM1. Cells were blocked with goat serum before testing with this LFA-1 Ligand Antibody.