Integrin Beta 4 Antibody 7G10D2 CD104 Monoclonal

	Catalog No	Formulation	Size	Price (USD)
	RQ7118	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

Integrin Beta 4 Antibody 7G10D2 in Human A431 Epidermoid Carcinoma Cells IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells with Integrin Beta 4 Antibody clone 7G10D2 (green) and DAPI nuclear stain (blue). HIER was performed by steaming the sample in pH 6 citrate buffer for 20 min. Green ITGB4 signal is distributed prominently around the cells, with strong peripheral and membrane-associated staining contrasting with the blue nuclei. This localization is consistent with the cell-surface and adhesion-associated distribution of integrin beta 4 and demonstrates detection using the CD104 Monoclonal Antibody.

Integrin Beta 4 Antibody 7G10D2 Western Blot of A549, PC-3 and Caco-2 Cells. Western blot testing of human A549 (lane 1), PC-3 (lane 2), and Caco-2 (lane 3) cell lysates with Integrin Beta 4 Antibody clone 7G10D2. The predicted molecular weight of ITGB4 is approximately 200 kDa. A prominent high-molecular-weight band is detected near the expected position in the tested lysates, supporting recognition of full-length integrin beta 4. The endogenous cell-line validation demonstrates target detection using the CD104 Monoclonal Antibody.

Integrin Beta 4 Antibody 7G10D2 in Human MCF7 Breast Cancer Cells FACS. Flow cytometry testing of human MCF7 breast cancer cells with Integrin Beta 4 Antibody clone 7G10D2 at 1 ug/million cells following blocking with goat sera. Cells alone are shown in red, isotype control in green, and antibody-treated cells in blue. The blue population displays a clear rightward fluorescence shift relative to both controls, demonstrating robust detection of cell-associated integrin beta 4 by the CD104 Monoclonal Antibody.

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Integrin Beta 4 Antibody 7G10D2 in Human A431 Epidermoid Carcinoma Cells IF. Immunofluorescence staining of FFPE human A431 epidermoid carcinoma cells with Integrin Beta 4 Antibody clone 7G10D2 (green) and DAPI nuclear stain (blue). HIER was performed by steaming the sample in pH 6 citrate buffer for 20 min. Green ITGB4 signal is distributed prominently around the cells, with strong peripheral and membrane-associated staining contrasting with the blue nuclei. This localization is consistent with the cell-surface and adhesion-associated distribution of integrin beta 4 and demonstrates detection using the CD104 Monoclonal Antibody.

Integrin Beta 4 Antibody 7G10D2 Western Blot of A549, PC-3 and Caco-2 Cells. Western blot testing of human A549 (lane 1), PC-3 (lane 2), and Caco-2 (lane 3) cell lysates with Integrin Beta 4 Antibody clone 7G10D2. The predicted molecular weight of ITGB4 is approximately 200 kDa. A prominent high-molecular-weight band is detected near the expected position in the tested lysates, supporting recognition of full-length integrin beta 4. The endogenous cell-line validation demonstrates target detection using the CD104 Monoclonal Antibody.

Integrin Beta 4 Antibody 7G10D2 in Human MCF7 Breast Cancer Cells FACS. Flow cytometry testing of human MCF7 breast cancer cells with Integrin Beta 4 Antibody clone 7G10D2 at 1 ug/million cells following blocking with goat sera. Cells alone are shown in red, isotype control in green, and antibody-treated cells in blue. The blue population displays a clear rightward fluorescence shift relative to both controls, demonstrating robust detection of cell-associated integrin beta 4 by the CD104 Monoclonal Antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b
Clone Name	7G10D2
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P16144
Applications	Western Blot : 0.5-1ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This Integrin Beta 4 Antibody 7G10D2 / CD104 Monoclonal Antibody is available for research use only.
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Description

Integrin Beta 4 Antibody 7G10D2 recognizes integrin beta 4, a transmembrane adhesion receptor subunit encoded by the ITGB4 gene and also designated CD104. ITGB4 is structurally distinct from other integrin beta chains and associates predominantly with integrin alpha 6 to form the alpha 6 beta 4 heterodimer. This receptor has important functions in epithelial cell attachment, hemidesmosome organization, and signaling between cells and the extracellular matrix.

Integrin beta 4 is a type I transmembrane glycoprotein distinguished by an exceptionally large cytoplasmic domain containing more than 1,000 amino acids. This intracellular region includes four fibronectin type III domains and multiple sites for interactions with cytoskeletal and signaling proteins. Its unusual architecture enables alpha 6 beta 4 to provide both stable mechanical adhesion and a platform for signaling events that influence cellular behavior.

Alpha 6 beta 4 is a major component of hemidesmosomes, specialized adhesion structures that connect epithelial cells to the underlying basement membrane. The extracellular portion of the receptor binds laminins, particularly laminin-332, while the ITGB4 cytoplasmic domain interacts with proteins such as plectin and BP230. These interactions connect the receptor complex to keratin intermediate filaments and help epithelial tissues withstand mechanical stress.

CD104 also participates in signaling associated with cell migration, proliferation, survival, and tissue remodeling. Phosphorylation and altered protein interactions within the large ITGB4 cytoplasmic domain can contribute to hemidesmosome disassembly and receptor redistribution. Changes in alpha 6 beta 4 expression and signaling have consequently been investigated in epithelial tumor progression, invasion, and other processes involving altered cell-matrix adhesion.

Pathogenic variants affecting ITGB4 can impair epithelial attachment and are associated with forms of junctional epidermolysis bullosa, including disease accompanied by pyloric atresia. These disorders demonstrate the importance of integrin beta 4 in maintaining stable epithelial-basement membrane adhesion and normal tissue integrity.

An Integrin Beta 4 Antibody such as clone 7G10D2 provides a monoclonal reagent for investigating ITGB4 expression and alpha 6 beta 4 biology, while a CD104 Monoclonal Antibody uses the established cluster-of-differentiation designation for studies of epithelial adhesion and integrin signaling.

For a broadly validated antibody targeting this protein, see our CD104 Antibody / ITGB4 Polyclonal Antibody page.

Application Notes

Optimal dilution of the Integrin Beta 4 Antibody 7G10D2 / CD104 Monoclonal Antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R29-K431) was used as the immunogen for the Integrin Beta 4 Antibody 7G10D2.

Storage

After reconstitution, the Integrin Beta 4 Antibody 7G10D2 can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

ITGB4 antibody, CD104 antibody, Beta 4 Integrin antibody, Integrin beta-4 subunit antibody, GP150 antibody, CD104 antigen antibody

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Integrin Beta 4 Antibody 7G10D2 / CD104 Monoclonal Antibody [clone 7G10D2] (RQ7118)

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