Integrin Alpha 3 Antibody / CD49c Polyclonal Antibody (RQ7004)

Integrin Alpha 3 Antibody by IHC in human lung adenocarcinoma. Immunohistochemistry of FFPE human lung adenocarcinoma tissue using Integrin Alpha 3 Antibody. Brown DAB immunoreactivity is prominent in tumor cells, with membranous and cytoplasmic staining outlining glandular structures. Hematoxylin counterstaining highlights blue nuclei. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes. This CD49c Polyclonal Antibody detects integrin alpha 3, a cell adhesion receptor involved in extracellular matrix interactions.

Integrin Alpha 3 Antibody by IHC in human colorectal adenocarcinoma. Immunohistochemistry of FFPE human colorectal adenocarcinoma tissue using Integrin Alpha 3 Antibody. Brown DAB staining is visible in malignant epithelial cells, with prominent membranous and cytoplasmic immunoreactivity along glandular structures. Surrounding stromal tissue shows comparatively weaker staining, while hematoxylin highlights blue nuclei. Antigen retrieval was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes. This CD49c Polyclonal Antibody recognizes integrin alpha 3, a laminin-binding cell adhesion receptor.

Integrin Alpha 3 Antibody by IHC in human esophageal squamous carcinoma. Immunohistochemistry of FFPE human esophageal squamous carcinoma tissue using Integrin Alpha 3 Antibody. Strong brown DAB immunoreactivity is visible throughout a prominent tumor cell cluster, with membranous and cytoplasmic staining. Surrounding stromal regions exhibit comparatively limited staining. Hematoxylin counterstaining highlights blue nuclei and tissue architecture. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer for 20 minutes. This CD49c Polyclonal Antibody detects integrin alpha 3 in malignant squamous epithelial cells.

Integrin Alpha 3 Antibody by IF/ICC in human A431 cells. Immunofluorescence staining of FFPE human A431 cells using Integrin Alpha 3 Antibody. Green fluorescence highlights integrin alpha 3 expression, with predominantly cytoplasmic and peripheral cellular staining. Nuclei are counterstained blue with DAPI. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes. This CD49c Polyclonal Antibody detects a transmembrane adhesion receptor involved in cell-matrix interactions and cellular migration.

Integrin Alpha 3 Antibody by WB in human U-87 MG and A431 cells. Western blot analysis of human U-87 MG (lane 1) and A431 (lane 2) cell lysates using Integrin Alpha 3 Antibody. Prominent immunoreactive bands are detected at approximately 130-150 kDa, consistent with the expected molecular weight range of glycosylated integrin alpha 3. The A431 sample shows an additional closely migrating band within this range. This CD49c Polyclonal Antibody recognizes ITGA3, a transmembrane glycoprotein involved in cell adhesion and extracellular matrix signaling.

Integrin Alpha 3 Antibody by FACS in human U-87 MG cells. Flow cytometry analysis of human U-87 MG cells using Integrin Alpha 3 Antibody at 1 ug per million cells following blocking with goat serum. The overlay histogram demonstrates a clear rightward fluorescence shift in antibody-stained cells (blue) compared with the isotype control (green) and unstained control (red). This CD49c Polyclonal Antibody detects integrin alpha 3 in U-87 MG cells, demonstrating immunoreactivity under the tested flow cytometry conditions.