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Home >> Antibodies >> Insulin receptor substrate 2 Antibody / IRS2

Insulin receptor substrate 2 Antibody / IRS2 (F54949)

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  Catalog No Formulation Size Price (USD)  
Image F54949-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54949-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human HeLa cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa but it may be observed at up to ~190 kDa due to phosphorylation.
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Western blot testing of human HeLa cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa but it may be observed at up to ~190 kDa due to phosphorylation.
Western blot testing of human HEK293 cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa but it may be observed at up to ~190 kDa due to phosphorylation.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Insulin receptor substrate 2 antibody (green) and anti-Actin (red).
IHC testing of FFPE human hepatocellular carcinoma tissue with Insulin receptor substrate 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q9Y4H2
Localization Cytoplasmic
Applications Western blot : 1:500-1:1000
Immunofluorescence : 1:10-1:50
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This Insulin receptor substrate 2 antibody is available for research use only.
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Description

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Insulin receptor substrate 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1309-1338 from the human protein was used as the immunogen for the Insulin receptor substrate 2 antibody.

Storage

Aliquot the Insulin receptor substrate 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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