Insulin Antibody IRDN/794 / Mouse Monoclonal [clone IRDN/794] (V2635)

Description

Insulin Antibody IRDN/794 recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

For a highly published insulin monoclonal with an alternate INS05 clone designation, see our Insulin Antibody 2D11-H5 / INS05 Antibody page.

Application Notes

Optimal dilution of the Insulin Antibody IRDN/794 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant INS protein was used as the immunogen for the Insulin Antibody IRDN/794.

Storage

Store the Insulin Antibody IRDN/794 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).