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Home >> Antibodies >> Insulin Antibody

Insulin Antibody [clone BCPH1] (V7133)

  Catalog No Formulation Size Price (USD)  
Image V7133-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V7133-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V7133SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V7133IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC testing of FFPE human pancreas stained with Insulin antibody (clone BCPH1).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name BCPH1
Purity Protein G affinity chromatography
UniProt P01308
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Insulin antibody is available for research use only.
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Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. [RefSeq]

Application Notes

Optimal dilution of the Insulin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant INS protein was used as the immunogen for the Insulin antibody.

Storage

Store the Insulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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