INPP5D Antibody / SHIP1 Polyclonal Antibody (RQ8586)

INPP5D Antibody WB Analysis of Wild-Type and INPP5D Knockout Human THP-1 Cells. Western blot analysis of human THP-1 wild-type (WT, lane 1) and INPP5D knockout (KO, lane 2) whole-cell lysates using SHIP1 Polyclonal Antibody at 0.5 ug/ml. Each lane contained 30 ug of total protein separated by 8% SDS-PAGE under reducing conditions. A prominent immunoreactive band near the 130 kDa molecular weight marker in WT lysate is substantially reduced in the KO sample, supporting target-dependent recognition of INPP5D. The observed migration is consistent with the predicted molecular weight of approximately 133 kDa. Beta-Tubulin serves as the loading control.

INPP5D Antibody WB Analysis of Human Raji, THP-1 and K562 Cell Lysates. Western blot analysis of human Raji (lane 1), THP-1 (lane 2), and K562 (lane 3) cell lysates using SHIP1 Polyclonal Antibody. An immunoreactive band near 145 kDa is detected most prominently in THP-1 lysate, with a weaker corresponding band in Raji lysate and no comparably prominent band in K562 lysate at the displayed exposure. The observed migration is above the predicted molecular weight of approximately 133 kDa for INPP5D.

INPP5D Antibody FACS Analysis of Human THP-1 Cells. Intracellular flow cytometry analysis of fixed and permeabilized human THP-1 cells using SHIP1 Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. The blue histogram represents antibody-stained cells, the green histogram represents the isotype control, and the red histogram represents unstained cells. The antibody-stained population exhibits a rightward fluorescence shift relative to the isotype control, supporting intracellular detection of INPP5D under the tested conditions.