INI1 Antibody SMARCB1/3984 | BAF47 Monoclonal Antibody V9617

	Catalog No	Formulation	Size	Price (USD)
	V9617-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9617-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9617SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

INI1 Antibody SMARCB1/3984 by IHC in Epithelioid Sarcoma Immunohistochemistry of FFPE human epithelioid sarcoma sections using INI1 Antibody clone SMARCB1/3984. Strong, predominantly nuclear staining is observed across numerous tumor cells, consistent with the expected nuclear localization of SMARCB1/BAF47. This BAF47 Monoclonal Antibody produces well-defined nuclear labeling with limited background staining. Sections underwent heat-induced epitope retrieval in pH 9 Tris-EDTA buffer before staining.

IHC staining of FFPE human testis tissue with INI1 antibody (clone SMARCB1/3984) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

INI1 Antibody SMARCB1/3984 Protein Microarray Specificity Protein microarray analysis of INI1 Antibody clone SMARCB1/3984 using a HuProt array containing more than 19,000 full-length human proteins. INI1 produces the dominant signal with a Z-score of approximately 90, substantially exceeding the next-ranked proteins. The pronounced separation between the intended target and other array proteins supports the target specificity of this BAF47 Monoclonal Antibody.

INI1 Antibody SMARCB1/3984 by IHC in Human Testis Immunohistochemistry of FFPE human testis sections using INI1 Antibody clone SMARCB1/3984 at 2 ug/ml. Strong nuclear staining is evident within the seminiferous tubules, while the PBS negative-control inset shows minimal background signal. The staining pattern demonstrates distinct nuclear labeling by this BAF47 Monoclonal Antibody. Sections underwent heat-induced epitope retrieval in pH 9 Tris-EDTA buffer before staining.

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INI1 Antibody SMARCB1/3984 by IHC in Epithelioid Sarcoma Immunohistochemistry of FFPE human epithelioid sarcoma sections using INI1 Antibody clone SMARCB1/3984. Strong, predominantly nuclear staining is observed across numerous tumor cells, consistent with the expected nuclear localization of SMARCB1/BAF47. This BAF47 Monoclonal Antibody produces well-defined nuclear labeling with limited background staining. Sections underwent heat-induced epitope retrieval in pH 9 Tris-EDTA buffer before staining.

INI1 Antibody SMARCB1/3984 by IHC in Epithelioid Sarcoma Immunohistochemistry of FFPE human epithelioid sarcoma sections using INI1 Antibody clone SMARCB1/3984. At higher magnification, prominent nuclear labeling is evident within the tumor cells with comparatively limited cytoplasmic staining. The BAF47 Monoclonal Antibody provides clear visualization of the characteristic nuclear staining pattern. Sections underwent heat-induced epitope retrieval in pH 9 Tris-EDTA buffer before staining.

IHC staining of FFPE human testis tissue with INI1 antibody (clone SMARCB1/3984) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

INI1 Antibody SMARCB1/3984 Protein Microarray Specificity Protein microarray analysis of INI1 Antibody clone SMARCB1/3984 using a HuProt array containing more than 19,000 full-length human proteins. INI1 produces the dominant signal with a Z-score of approximately 90, substantially exceeding the next-ranked proteins. The pronounced separation between the intended target and other array proteins supports the target specificity of this BAF47 Monoclonal Antibody.

INI1 Antibody SMARCB1/3984 by IHC in Human Testis Immunohistochemistry of FFPE human testis sections using INI1 Antibody clone SMARCB1/3984 at 2 ug/ml. Strong nuclear staining is evident within the seminiferous tubules, while the PBS negative-control inset shows minimal background signal. The staining pattern demonstrates distinct nuclear labeling by this BAF47 Monoclonal Antibody. Sections underwent heat-induced epitope retrieval in pH 9 Tris-EDTA buffer before staining.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	SMARCB1/3984
Purity	Protein A/G affinity
UniProt	Q12824
Localization	Nucleus
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This INI1 Antibody SMARCB1/3984 / BAF47 Monoclonal Antibody is available for research use only.
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Description

INI1 Antibody SMARCB1/3984 recognizes integrase interactor 1, a nuclear protein encoded by the SMARCB1 gene and also widely known as BAF47, SNF5 and hSNF5. SMARCB1 is a core component of the SWI/SNF family of ATP-dependent chromatin-remodeling complexes, which regulate access of transcriptional machinery and other nuclear factors to chromatin. Through these complexes, SMARCB1 participates in transcriptional regulation, cellular differentiation, proliferation and maintenance of normal developmental programs.

SMARCB1 functions as an important tumor suppressor. Biallelic inactivation of SMARCB1 is a defining molecular alteration in malignant rhabdoid tumors and is also associated with several other tumor types. Loss of functional SMARCB1 can disrupt normal SWI/SNF complex activity and alter transcriptional programs involved in cell-cycle regulation and differentiation. These properties have made INI1 expression an important subject of research in tumor biology and molecular pathology.

The protein is commonly referred to as INI1 because it was originally identified through its interaction with HIV-1 integrase. It is also known as BAF47, reflecting its association with the BAF form of the mammalian SWI/SNF complex, and as SNF5 or hSNF5 because of its relationship to the yeast SNF5 chromatin-remodeling protein. These names remain widely used in research and pathology literature, although SMARCB1 is the official gene symbol.

Altered or absent INI1 expression is particularly associated with malignant rhabdoid tumor, epithelioid sarcoma and several other uncommon neoplasms. Conversely, preserved nuclear expression can provide useful biological context when studying tumors that enter the differential diagnosis of SMARCB1-deficient neoplasms. Research into SMARCB1 loss has also contributed to a broader understanding of how disruption of SWI/SNF chromatin-remodeling complexes contributes to oncogenesis.

Because SWI/SNF complexes influence numerous transcriptional and developmental pathways, SMARCB1 is studied beyond its role as a tumor marker. Investigations of the protein encompass chromatin organization, epigenetic regulation, lineage specification, cell-cycle control and mechanisms of tumor suppression. NSJ Bioreagents offers this BAF47 Monoclonal Antibody for research involving INI1 and its associated molecular pathways. An INI1 Antibody can therefore be useful for investigating SMARCB1/BAF47 expression and the biological consequences of altered SWI/SNF chromatin-remodeling activity.

Explore additional research tools in our Cancer Marker Antibodies collection.

Application Notes

Optimal dilution of the INI1 Antibody SMARCB1/3984 / BAF47 Monoclonal Antibody should be determined by the researcher.

Immunogen

A portion of amino acids 65-184 was used as the immunogen for the INI1 Antibody SMARCB1/3984.

Storage

Aliquot the INI1 Antibody SMARCB1/3984 and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Integrase Interactor 1 antibody, SMARCB1 antibody, BAF47 antibody, SNF5 antibody, INI-1 antibody, hSNF5 antibody

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INI1 Antibody SMARCB1/3984 / BAF47 Monoclonal Antibody [clone SMARCB1/3984] (V9617)

Description

Application Notes

Immunogen

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