Inhibin Alpha Antibody / INHA Polyclonal Antibody (RQ5822)

Inhibin Alpha Antibody Detection in Human U-2 OS Osteosarcoma Cells by IF. Immunofluorescence staining of FFPE human U-2 OS osteosarcoma cells using Inhibin Alpha Antibody shows distinct green cytoplasmic staining, while nuclei are counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 min before testing. The predominantly cytoplasmic distribution demonstrates detection of INHA using this INHA Polyclonal Antibody.

Inhibin Alpha Antibody Staining in Rat Testis by IHC. Immunohistochemistry staining of FFPE rat testis using Inhibin Alpha Antibody shows brown cytoplasmic labeling within seminiferous tubules, with heterogeneous staining intensity among cells. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The intratubular staining demonstrates detection of INHA in rat testis sections using this INHA Polyclonal Antibody.

Inhibin Alpha Antibody Detection in Human, Rat and Mouse Lysates by WB. Western blot analysis using Inhibin Alpha Antibody shows a predominant band at approximately 40-45 kDa across five human and rodent samples. Lane 1: human K562; lane 2: human HeLa; lane 3: rat brain; lane 4: mouse spleen; lane 5: mouse SP2/0. The consistent major band across all five lanes is compatible with the INHA precursor and demonstrates detection in human, rat, and mouse lysates using this INHA Polyclonal Antibody.

Inhibin Alpha Antibody Detection in Human SiHa Cervical Cancer Cells by FACS. Flow cytometry analysis of human SiHa cervical cancer cells using Inhibin Alpha Antibody at 1 ug/million cells shows a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat sera before staining. The separation of the blue population from both controls demonstrates detection of INHA in SiHa cells using this INHA Polyclonal Antibody.