Indoleamine 2,3-Dioxygenase 2 Antibody / Tumor Immune Microenvironment Enzyme Antibody (RQ6383)

Indoleamine 2,3-Dioxygenase 2 Antibody Liver Cancer IHC. Immunohistochemistry analysis of FFPE human liver carcinoma tissue using Indoleamine 2,3-Dioxygenase 2 Antibody shows cytoplasmic staining in tumor cells with heterogeneous intensity across the tumor field, consistent with IDO2 / INDOL1 localization as a tryptophan metabolism enzyme. The staining is observed within tumor epithelial regions and surrounding microenvironmental cells, supporting involvement in tumor-associated immune regulation. Hematoxylin counterstain provides nuclear contrast and tissue architecture. HIER: boil FFPE tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

Indoleamine 2,3-Dioxygenase 2 Antibody Multi-Species WB. Western blot analysis of 1) rat liver, 2) mouse liver, and 3) monkey kidney tissue lysates using Indoleamine 2,3-Dioxygenase 2 Antibody detects a band at approximately 47 kDa, consistent with the expected molecular weight of IDO2 / INDOL1. The banding pattern across multiple species supports detection of this tryptophan metabolism enzyme, aligning with its role in kynurenine pathway activity and immune-associated metabolic regulation.

Indoleamine 2,3-Dioxygenase 2 Antibody K562 Cell FACS. Flow cytometry analysis of human K562 cells using Indoleamine 2,3-Dioxygenase 2 Antibody shows a clear rightward shift of the blue histogram relative to the isotype control (green) and unstained cells (red), indicating detection of IDO2 / INDOL1 expression. The intracellular signal distribution supports specific antibody binding to this tryptophan metabolism enzyme involved in immune-associated metabolic regulation.