INDOL1 Antibody / Tryptophan Metabolism Enzyme Antibody [clone IDO2/2638] (V9591)

INDOL1 Antibody HuProt Microarray Specificity. Protein microarray analysis using INDOL1 Antibody (clone IDO2/2638) demonstrates highly specific binding to IDO2 / INDOL1 (Indoleamine 2,3-dioxygenase 2), with the target protein ranked as the top hit and showing a strong Z score with clear separation from all other proteins on the array. Signal intensity decreases sharply for non-target proteins, supporting selective recognition with minimal off-target interaction. Z score represents the strength of signal in standard deviations above the mean of all array signals, while S score reflects the separation between ranked targets and provides a measure of relative specificity.

INDOL1 Antibody Placental Tissue IHC. Immunohistochemistry analysis of FFPE human placental tissue using INDOL1 Antibody (clone IDO2/2638) shows cytoplasmic staining in trophoblast cells, consistent with INDOL1 / IDO2 (Indoleamine 2,3-dioxygenase 2) localization as a tryptophan metabolism enzyme. The staining highlights placental epithelial structures with strong signal, while surrounding stromal regions display comparatively lower intensity. Negative control inset using PBS in place of primary antibody confirms minimal non-specific background staining. Hematoxylin counterstain provides nuclear contrast and tissue architecture. Antibody incubation was performed at 2 ug/ml in PBS for 30 min at RT. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

INDOL1 Antibody NIH 3T3 Cell WB. Western blot analysis of mouse NIH 3T3 cell lysate using INDOL1 Antibody (clone IDO2/2638) detects a band at approximately 47 kDa, consistent with the expected molecular weight of INDOL1 / IDO2 (Indoleamine 2,3-dioxygenase 2). The observed signal supports detection of this tryptophan metabolism enzyme in fibroblast-derived cells, aligning with its role in kynurenine pathway activity and immune-related metabolic regulation.