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Home >> Antibodies >> ILKAP Antibody

ILKAP Antibody (F54838)

  Catalog No Formulation Size Price (USD)  
Image F54838-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54838-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human skeletal muscle tissue with ILKAP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HEK293 cell lysate with ILKAP antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ILKAP antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9H0C8
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This ILKAP antibody is available for research use only.
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Description

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the ILKAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 243-270 from the human protein was used as the immunogen for the ILKAP antibody.

Storage

Aliquot the ILKAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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