ILF2 Antibody | Interleukin enhancer-binding factor 2

	Catalog No	Formulation	Size	Price (USD)
	RQ8536	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	439
Formulation

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IHC staining of FFPE human rectum adenocarcinoma tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid papillary carcinoma tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous carcinoma tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with ILF2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human HeLa cells with ILF2 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human K562, 2) human Jurkat, 3) human HeLa, 4) monkey COS-7, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with ILF2 antibody. Predicted molecular weight ~43 kDa.

Flow cytometry testing of fixed and permeabilized human JK cells with ILF2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ILF2 antibody.

Availability	1-3 days
Species Reactivity	Human, Mouse, Rat, Monkey
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q12905
Localization	Nuclear, cytoplasmic
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml
Limitations	This ILF2 antibody is available for research use only.
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Description

Interleukin enhancer-binding factor 2 is a protein that in humans is encoded by the ILF2 gene. The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.

Application Notes

Optimal dilution of the ILF2 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids G21-K356) was used as the immunogen for the ILF2 antibody.

Storage

After reconstitution, the ILF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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ILF2 Antibody / Interleukin enhancer-binding factor 2 / NF45 (RQ8536)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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