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Home >> Antibodies >> IL3 Antibody

IL3 Antibody (R30148)

  Catalog No Formulation Size Price (USD)  
Image R30148 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Western blot testing of IL3 antibody and Lane 1: PC-12; 2: NRK; 3: RH35; The glycoprotein is routinely visualized between 18~35 kDa.
Western blot testing of IL3 antibody and recombinant protein (0.5ng)
Availability 1-3 business days
Species Reactivity Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 24495
Applications Western Blot : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (rat protein tested); request BSA free format for coating
Limitations This IL3 antibody is available for research use only.
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Description

Interleukin 3 is a glycoprotein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

For a monoclonal clone with IHC and protein microarray specificity validation, see our Interleukin 3 Antibody IL3/4004 / IL3 Antibody page.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the IL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Rat partial recombinant protein (AA 28-166) was used as the immunogen for this IL3 antibody.

Storage

After reconstitution, the IL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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