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Home >> Antibodies >> IL2RB Antibody

IL2RB Antibody (RQ6188)

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  Catalog No Formulation Size Price (USD)  
Image RQ6188 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with IL2RB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with IL2RB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human appendicitis tissue with IL2RB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HEK293, 3) K562, 4) ThP-1, 5) rat PC-12, 6) mouse intestine and 7) mouse RAW264.7 lysate with IL2RB antibody. Expected molecular weight ~61 kDa (unmodified), 70-75 kDa (glycosylated).
Flow cytometry testing of human U-2 OS cells with IL2RB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL2RB antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide
UniProt P14784
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This IL2RB antibody is available for research use only.
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Description

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. [UniProt]

Application Notes

Optimal dilution of the IL2RB antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids R268-V551) was used as the immunogen for the IL2RB antibody.

Storage

After reconstitution, the IL2RB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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