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Home >> Antibodies >> IL1 beta Antibody / IL1B

IL1 beta Antibody / IL1B [clone IL1B/3993] (V9593)

  Catalog No Formulation Size Price (USD)  
Image V9593-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9593-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9593SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC staining of FFPE human adrenal gland with IL1B antibody (clone IL1B/3993). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with IL1B antibody (clone IL1B/3993). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IL1B antibody (clone IL1B/3993). These results demonstrate the foremost specificity of the IL1B/3993 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name IL1B/3993
Purity Protein A/G affinity
UniProt P01584
Localization Cytoplasm, Extracellular (secreted)
Applications ELISA (order BSA-free format for coating) :
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This IL1B antibody is available for research use only.
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Description

The IL1 beta cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Application Notes

Optimal dilution of the IL1B antibody should be determined by the researcher.

Immunogen

A recombinant protein fragment was used as the immunogen for the IL1B antibody.

Storage

Aliquot the IL1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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