Interleukin 7 Antibody IL7/4013 / IL-7 Monoclonal Antibody [clone IL7/4013] (V9629)

Interleukin 7 Antibody IL7/4013 by FACS in Human U-87 MG Cells. Flow cytometry analysis of PFA-fixed human U-87 MG glioblastoma cells using Interleukin 7 Antibody IL7/4013. The blue histogram representing antibody staining shows a pronounced rightward fluorescence shift relative to the red isotype control histogram. The two populations are clearly separated, with antibody-treated cells exhibiting greater fluorescence intensity. The IL-7 Monoclonal Antibody demonstrates immunoreactivity in fixed U-87 MG cells under the tested conditions.

Interleukin 7 Antibody IL7/4013 by IHC in Human Colon Carcinoma. Immunohistochemistry of FFPE human colon carcinoma tissue using Interleukin 7 Antibody IL7/4013. Brown cytoplasmic staining is observed in malignant epithelial cells forming irregular glandular structures, with variable staining intensity across the tissue section. The IL-7 Monoclonal Antibody highlights the tumor cell cytoplasm, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

Interleukin 7 Antibody IL7/4013 by IF in Human U-87 MG Cells. Immunofluorescence staining of PFA-fixed human U-87 MG glioblastoma cells using Interleukin 7 Antibody IL7/4013. Green fluorescence is observed throughout the cytoplasm, with a granular staining pattern and variable intensity among individual cells. The IL-7 Monoclonal Antibody produces prominent cytoplasmic staining, while red phalloidin fluorescence highlights filamentous actin and cellular morphology. The merged image demonstrates the distribution of antibody immunoreactivity within the cells.

Interleukin 7 Antibody IL7/4013 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Interleukin 7 Antibody IL7/4013 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 130-150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed near 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The IL-7 Monoclonal Antibody demonstrates the expected electrophoretic profile of a purified mouse monoclonal antibody preparation.

Interleukin 7 Antibody IL7/4013 by Protein Microarray Analysis. HuProt protein microarray analysis of Interleukin 7 Antibody IL7/4013 against more than 19,000 full-length human proteins. The graph displays a dominant binding signal for IL-7, clearly separated from the remaining proteins on the array. The IL-7 Monoclonal Antibody demonstrates preferential recognition of its intended target, supporting its specificity in this protein microarray assay. The Z-score represents the strength of the binding signal relative to the array background, while the S-score reflects the difference between the intended target and the next-highest ranked protein.