IL-7 Antibody IL7/4012 / Interleukin 7 Monoclonal Antibody [clone IL7/4012] (V9628)

IL-7 Antibody IL7/4012 by IF in Human U-87 MG Cells. Immunofluorescence staining of PFA-fixed human U-87 MG glioblastoma cells using IL-7 Antibody IL7/4012. Green fluorescence is distributed throughout the cytoplasm, with granular staining and variable intensity among individual cells. Red phalloidin staining highlights filamentous actin and cellular morphology. The Interleukin 7 Monoclonal Antibody produces a predominantly cytoplasmic staining pattern, with little fluorescence observed in the nuclear regions.

IL-7 Antibody IL7/4012 by IHC in Human Adrenal Gland. Immunohistochemistry of FFPE human adrenal gland tissue using IL-7 Antibody IL7/4012. Brown cytoplasmic staining is observed in groups of adrenal cells, with variable staining intensity across the tissue section. The Interleukin 7 Monoclonal Antibody highlights numerous cells exhibiting moderate to strong cytoplasmic immunoreactivity, while other regions show comparatively weaker staining. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

IL-7 Antibody IL7/4012 by FACS in Human U-87 MG Cells. Flow cytometry analysis of PFA-fixed human U-87 MG cells using IL-7 Antibody IL7/4012. The blue histogram representing antibody staining exhibits a pronounced rightward fluorescence shift relative to the red isotype control histogram. The two populations show clear separation, with antibody-treated cells displaying substantially greater fluorescence intensity. The Interleukin 7 Monoclonal Antibody demonstrates immunoreactivity in fixed U-87 MG cells under the tested conditions.

IL-7 Antibody IL7/4012 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free IL-7 Antibody IL7/4012 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating at approximately 130-150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed near 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The Interleukin 7 Monoclonal Antibody demonstrates the expected electrophoretic profile of a purified mouse monoclonal antibody preparation.

IL-7 Antibody IL7/4012 by Protein Microarray Analysis. HuProt protein microarray analysis of IL-7 Antibody IL7/4012 against more than 19,000 full-length human proteins. The graph displays the ranked antibody-binding signals, with the strongest signal clearly separated from the remaining proteins. The Interleukin 7 Monoclonal Antibody demonstrates preferential recognition of its intended target in this protein microarray assay. The Z-score represents signal intensity relative to the array background, while the S-score reflects the separation between the intended target and the next-highest ranked protein.