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Home >> Antibodies >> IL-4 Antibody

IL-4 Antibody (R30841)

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  Catalog No Formulation Size Price (USD)  
Image R30841 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of IL-4 antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: human CCRF-CEM cell lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.
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Western blot testing of IL-4 antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: human CCRF-CEM cell lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.
IHC-P: IL-4 antibody testing of human tonsil tissue
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P05112
Localization Secreted
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This IL-4 antibody is available for research use only.
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Description

Interleukin-4, also knowns as a B-cell stimulatory factor 1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human Interleukin-4 (AATVLRQFYSHHEKDTR) was used as the immunogen for this IL-4 antibody.

Storage

After reconstitution, the IL-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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