IL-31 Antibody / Interleukin 31 Polyclonal Antibody (RQ5303)

IL-31 Antibody in Human Tonsil Lymphoid and Epithelial Regions by IHC. Immunohistochemistry of FFPE human tonsil sections using IL-31 Antibody at 2 ug/ml. The image shows heterogeneous predominantly cytoplasmic brown staining across lymphoid cells, with stronger immunoreactivity visible in epithelial and stromal structures toward the tissue periphery. Dense lymphoid regions show comparatively weaker staining, producing clear regional contrast. HIER was performed using EDTA buffer at pH 8.0. This staining demonstrates tissue localization using the Interleukin 31 Polyclonal Antibody.

IL-31 Antibody in Human Tonsil Lymphoid Follicles by IHC. Immunohistochemistry of FFPE human tonsil sections using IL-31 Antibody at 2 ug/ml. The image shows heterogeneous cytoplasmic immunoreactivity within the lymphoid tissue, with stronger brown staining in lighter interfollicular and follicular cell populations and comparatively weak staining within densely packed lymphocyte-rich regions. This second field provides a complementary view of IL-31 distribution within human tonsil. HIER was performed using EDTA buffer at pH 8.0, supporting tissue-based validation of the Interleukin 31 Polyclonal Antibody.

IL-31 Antibody in Human, Rat and Mouse Samples by WB. Western blot analysis using IL-31 Antibody with 30 ug of reduced lysate per lane. Lane 1: human K562 cells; lane 2: human SH-SY5Y cells; lane 3: human Caco-2 cells; lane 4: rat testis; lane 5: rat PC-12 cells; lane 6: mouse testis; lane 7: mouse RAW264.7 cells. A strong band is detected at approximately 30-35 kDa across all seven samples, compared with the calculated IL-31 molecular weight of 18.2 kDa. The higher apparent molecular weight may reflect post-translational modification and/or migration characteristics of endogenous IL-31. The result demonstrates cross-species detection with this Interleukin 31 Polyclonal Antibody.