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Home >> Antibodies >> IL-25 Antibody / IL-17E

IL-25 Antibody / IL-17E [clone IL25/625] (V9633)

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  Catalog No Formulation Size Price (USD)  
Image V9633-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9633-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9633SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human colon carcinoma tissue with IL-25 antibody (clone IL25/625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon carcinoma tissue with IL-25 antibody (clone IL25/625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adrenal gland tissue with IL-25 antibody (clone IL25/625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free IL-25 antibody (clone IL25/625) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name IL25/625
Purity Protein A/G affinity
UniProt Q9H293
Localization Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This IL-25 antibody is available for research use only.
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Description

Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. They exert their biological effects through the binding of membrane-bound receptors which, in turn, initiate signal transduction cascades and elicit physiological changes in their target cell. Interleukin-17 (IL-17) and its cognate receptor, IL-17R, are an example of such a cytokine receptor pair. Originally identified as a rodent cDNA termed CTLA8, IL-17 is capable of inducing the secretion of IL-6 and IL-8 and augmenting the expression of ICAM-1 in human fibroblast cultures. The IL-17 protein exhibits a striking degree of homology with the HSV-13 protein which mimics its function. The IL-17 receptor is a type I transmembrane protein 864 amino acids in length that is highly expressed in spleen and kidney.

Application Notes

Optimal dilution of the IL-25 antibody should be determined by the researcher.

Immunogen

A recombinant protein fragment was used as the immunogen for the IL-25 antibody.

Storage

Aliquot the IL-25 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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