Interleukin 18 Antibody / IL-18 Polyclonal Antibody (R31439)

Interleukin 18 Antibody Detection in Human A549 Lung Carcinoma Cells IF. Immunofluorescence staining of FFPE human A549 lung carcinoma cells using Interleukin 18 Antibody shows red IL-18 signal concentrated in a punctate cytoplasmic and perinuclear pattern, while beta tubulin staining is shown in green. HIER was performed by steaming sections in pH 6 citrate buffer for 20 min. The distinct IL-18 signal demonstrates detection in A549 cells using this IL-18 Polyclonal Antibody.

Interleukin 18 Antibody Staining in Human Tonsil IHC. Immunohistochemistry staining of FFPE human tonsil tissue using Interleukin 18 Antibody shows heterogeneous brown staining in scattered cells distributed throughout the lymphoid tissue, with comparatively little staining in surrounding cells. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The staining demonstrates detection of IL-18 in human tonsil using this IL-18 Polyclonal Antibody.

Interleukin 18 Antibody Detects IL-18 in Human HeLa and HepG2 Cells WB. Western blot analysis of human HeLa (lane 1) and HepG2 (lane 2) cell lysates using Interleukin 18 Antibody shows strong, discrete bands at approximately 20 kDa in both samples, with minimal visible background. The observed molecular weight falls within the expected 17-24 kDa range for IL-18 and is compatible with processed IL-18 species. The consistent signal across both human cell lines demonstrates detection of IL-18 using this IL-18 Polyclonal Antibody.