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Home >> Antibodies >> IL-10 Antibody

IL-10 Antibody (R30421)

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  Catalog No Formulation Size Price (USD)  
Image R30421 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of IL-10 antibody and Lane 1: Recombinant human IL-10 protein 10ng; 2: 5ng; 3: 2.5ng
Western blot testing of IL-10 antibody and Lane 1: Recombinant human IL-10 protein 10ng; 2: 5ng; 3: 2.5ng
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P22301
Applications Western blot : 0.5-1ug/ml
Limitations This IL-10 antibody is available for research use only.
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Description

Interleukin-10, also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse il10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the IL-10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human Interleukin-10 (DAFSRVKTFFQMKDQ) was used as the immunogen for this IL-10 antibody.

Storage

After reconstitution, the IL-10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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