Interleukin-1 Alpha Antibody / IL1A Antibody (F49920)

Interleukin-1 Alpha Antibody in Human Esophagus IHC. Immunohistochemistry of paraffin-embedded human esophagus tissue using Interleukin-1 Alpha Antibody at 1:500 demonstrates weak-to-moderate cytoplasmic staining in the squamous epithelium and additional staining within surrounding tissue structures, while nuclei remain predominantly counterstained blue. Testing was performed on the Leica BOND RXm with primary antibody incubation for 1 hour at room temperature. The staining demonstrates detection of interleukin 1 alpha by this IL1A Antibody in human esophagus tissue.

Interleukin-1 Alpha Antibody Detects Recombinant Human IL1A WB. Western blot analysis of 20 ng recombinant human IL-1 alpha protein using Interleukin-1 Alpha Antibody at 1:2000 detects a strong, discrete band at approximately 21-22 kDa with minimal background. The observed band represents the recombinant IL1A preparation used in this assay and demonstrates direct recognition of human interleukin 1 alpha by this IL1A Antibody under Western blot conditions.

Interleukin-1 Alpha Antibody Detects IL1A in HepG2 Cells WB. Western blot analysis of human HepG2 cell lysate using Interleukin-1 Alpha Antibody detects a distinct band at approximately 34-36 kDa, close to the expected size of the approximately 31 kDa IL1A precursor. The figure does not show a prominent band corresponding to the approximately 17 kDa mature cytokine. This IL1A Antibody therefore primarily detects a species consistent with precursor IL1A under the conditions shown.

Interleukin-1 Alpha Antibody Detects IL1A in Ramos B Cells FACS. Flow cytometry analysis of fixed and permeabilized human Ramos B cells using Interleukin-1 Alpha Antibody at 1:25 demonstrates a strong rightward fluorescence shift in the antibody-stained population (green) relative to the rabbit IgG1 isotype control (blue). Cells were fixed with 2% paraformaldehyde, permeabilized with 90% methanol for 10 min, blocked with 2% BSA, and incubated with primary antibody for 60 min at 37oC. DyLight 488-conjugated goat anti-rabbit IgG at 1:200 was used for detection. The clear separation of the populations demonstrates intracellular IL1A detection by this IL1A Antibody.

Interleukin-1 Alpha Antibody Detects IL1A in Jurkat T Cells FACS. Flow cytometry analysis of fixed and permeabilized human Jurkat T cells using Interleukin-1 Alpha Antibody at 1:25 demonstrates a strong rightward fluorescence shift in the antibody-stained population (green) relative to the rabbit IgG1 isotype control (blue), with minimal overlap between the populations. Cells were fixed with 2% paraformaldehyde, permeabilized with 90% methanol for 10 min, blocked with 2% BSA, and incubated with primary antibody for 60 min at 37oC. DyLight 488-conjugated goat anti-rabbit IgG at 1:200 was used for detection. The pronounced separation demonstrates intracellular IL1A detection by this IL1A Antibody in Jurkat T cells.

Interleukin-1 Alpha Antibody Detects IL1A in Raji B Cells FACS. Flow cytometry analysis of fixed and permeabilized human Raji B cells using Interleukin-1 Alpha Antibody at 1:25 demonstrates a pronounced rightward fluorescence shift in the antibody-stained population (green) relative to the rabbit IgG1 isotype control (blue), with minimal overlap between the populations. Cells were fixed with 2% paraformaldehyde, permeabilized with 90% methanol for 10 min, blocked with 2% BSA, and incubated with primary antibody for 60 min at 37oC. DyLight 488-conjugated goat anti-rabbit IgG at 1:200 was used for detection. The clear population separation demonstrates intracellular IL1A detection by this IL1A Antibody in Raji B cells.