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Home >> Antibodies >> IKKG Antibody / NEMO

IKKG Antibody / NEMO (R32596)

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  Catalog No Formulation Size Price (USD)  
Image R32596 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with IKKG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with IKKG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
Flow cytometry testing of human PC-3 cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
Western blot testing of 1) rat heart and 2) human HepG2 lysate with IKKG antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q9Y6K9
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Limitations This IKKG antibody is available for research use only.
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Description

NF-kappa-B essential modulator (NEMO), also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (IKKg), is a protein that in humans is encoded by the IKBKG gene. This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Application Notes

Optimal dilution of the IKKG antibody should be determined by the researcher.

Immunogen

Amino acids 207-246 (QSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIK-human) were used as the immunogen for the IKKG antibody.

Storage

After reconstitution, the IKKG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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